|
Primary
Investigators |
Tom
L. Casavant
Ph.D.,
Center for Bioinformatics and Computational Biology |
Todd
Scheetz
Ph.D.,
Ophthalmology and Genetics |
Jian
Huang
Associate
Professor, Statistics & Actuarial Science and Program
in Public Health and Genetics |
Kevin
Knudtson
Ph.D,
Associate Research Scientist, DNA Facility |
|
|
|
The Univeristy of Iowa NHLBI Shared Microarray
Facility was begun in 2002 to facilitate the application of microarray technology
to fundamental, hypothesis-driven research for NHLBI
funded investigators at the University of Iowa. The facility accomplished
this goal through the enhancement of pre-existing microarray facilities.
This facility builds on the experience and expertise at the University of
Iowa to provide study design guidance, microarray services, analytical tools
and guidance in the interpretation of gene expression profiling results,
and appropriate education to the NHLBI-supported investigators in all relevant
aspects regarding microarray technology and its application.
This facility expands the functional Microarray Hybridization
Core currently established in Dr.
Soares' laboratory at the Univeristy of Iowa in 1999, and UI
DNA Core directed by Dr. Kevin Knudtson. This expansion has enabled
NHLBI funded investigators at the University of Iowa to take advantage of
microarray hybridization technology to further their ongoing NHLBI-supported
research. In addition to access to comprehensive non-redundant arrayed collections
of human, mouse, and rat cDNA clones, custom arrays, microarray hybridization
technology and expertise, these investigators are able to obtain all necessary
informatics and statistical support for processing, analysis/interpretation
and public dissemination/publication of their microarray hybridization data.
The informatics and statistical components of the proposed Shared Microarray
Facility are directed by Drs. Thomas
Casavant and Jian Huang,
respectivly.
Resources developed by this PGA:
The resources developed by this PGA consist of:
- cDNA glass slide microarrays derived from the following clone resources:
58,000 rat cDNAs from diverse tissues and developmental stages, 31,000
mouse cDNAs derived from multiple regions of the brain and developing
placenta, and 51000 human cDNAs derived from a number of tumors of high
prevalence in the USA and their normal counterparts, different regions
and development stages of the eye, from normal and cystic fibrosis airway
epithelia, and from full-term and developing placenta.
Support for custom array design, target preparation, and hybridization
provided to investigators.
- Informatics support including: experimenal design, clone/probe selection
for custom microarrays, microarray database infrastructure, analytical
tools, and knowledge discovery for probe sets.
- Statistical Support: data filtering and quality assessment, normalization,
and general and custom analyses.
|
|
Gene Lists |
|
Human (~34k)
|
|
Rat (~28k)
|
|
Mouse (~15k)
|
|
|
|
|
|
|
