Custom Sequence Annotation (CSA)



CSA Screenshots

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Process Options


Phred Parameters


EST Parameters


Repeat Masker Parameters


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Blast Options


Summary Page


Blast Summary


Clone Summary


Cluster Viewer

What is Custom Sequence Annotation (CSA)?

We have developed an EST sequence processing and annotation pipeline to process 3' and 5' sequence data derived from cDNA clones. This pipeline supports all types of cDNA libraries-single-tissue or pooled, normalized or subtracted from any species.

The pipeline consists of five major components:

  • Raw Data Gathering and Archival
  • Quality Assessment and Sequence Editing (Feature Identification)
  • Sequence Annotation
  • Novelty Assessment
  • Description or Submission to local and public databases.

UICluster Parallel Execution:

  • Uses MPI for communication (MPICH)
  • Distributes clusters evenly across multiple compute nodes
  • Global hash table searched in parallel
  • Communication performed after each input sequence is clustered


    This software may be downloaded free under the GNU Public License.

    UI Droppoint 1.0 Download